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KMID : 0363420100240010117
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Journal of Korean Oriental Pediatrics
2010 Volume.24 No. 1 p.117 ~ p.125
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Effect of Yukmijihwang-Tang Gamibang Aqua-acupuncture on the Recovery of Chondrocyte Phenotype
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Choi Won-Joo
Kim Jang-Hyun
Min Sang-Yeon
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Abstract
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Objectives: The purpose of this study is to examine the effect of chondrocyte re-differentiation in using ukmijihwang-Tang gamibang aqua-acupuncture.
Methods: In this study, chondrocytes were extracted from New Zealand white rabbit’s knee joint, and cultured in monolayer after collagenase treatment. In the third passage, after the mRNA transcript of the type II collagen significantly reduced, diluted Yukmijihwang-Tang gamibang were added to cultured of chondrocyte, and its effect on the mRNA expression of type II collagen was quantitatively evaluated.
Results: As a result of treatment with Yukmijihwang-Tang gamibang in vitro for 48 hours, the mRNA expression of type II collagen was up-regulated. In addition, the result of H&E-staining in vivo indicated that chondrocyte-like tissues were formed in repairing injured cartilages after 12 weeks of treatment with Yukmijihwang-Tang gamibang.
Conclusions: These results indicated that Yukmijihwang-Tang gamibang was effective on the recovery of chondrocyte phenotype, and could be used for cartilage regeneration in arthritic diseases. However, more clinical study of Oriental medical treatment for this case might be also needed.
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KEYWORD
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Chondrocyte, Yukmijihwang-Tang gamibang, Aqua-acupuncture, Type II collagen, Arthritis
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